In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. Dextrans in Aqueous Solution. Journal of Chemical Education, 49(8), 577-579. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. He, L.; Niemeyer, B. 2000;3(9):31826. Microporation techniques for enhanced delivery of therapeutic agents. Progress in Polymer Science, 30, 38-70. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. (1998). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. 1984;17(6):11703. 2016;47:15964. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. (2007). 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Epub 2012 Oct 18. Guner, A.; Kibarer, G. (2001). Journal of Physical Chemistry, 58(11), 953-957. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta eCollection 2018 Feb. Molecules. After 24h, the cumulative permeation of FITC-dextran M.W. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Determination of intrinsic viscosities of macromolecules and nanoparticles. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. The slope is a value and intercept is equal to the ln kMH value [47]. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. The Mark-Houwink. Corp. (Seoul, Korea). Please enable it to take advantage of the complete set of features! The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. Hydrodynamic radii ( RH) were determined by dynamic light scattering. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. ; Weisz, J. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. Solution properties of branched dextran. De Belder, A. N. (2001). Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Colloid Polym. Carbohydrate Polymers 72, 356-360. Polysaccharides-based nanoparticles as drug delivery systems. Webdextran hydrodynamic radius. pp13-21. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Carbohydrate Polymers 95 360-365. Expert Opin Drug Deliv. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. The term f/f0 is sometimes denoted as P, Perrin constant. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Catiker, E.; Guner, A. ; Garca de , J. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. J Control Release. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Advances in chemistry series Washington, DC: American Chemical Society., pp. Sachdeva V, Banga AK. (1999). Ahn, G.Y., Eo, HS., Kim, D. et al. 8. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. ; Picout, D.R. Increased TEWL values are evidence of micropores formation. Terms and Conditions, In addition, the permeation rate was higher in pig skin than in human skin. HHS Vulnerability Disclosure, Help Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Journal of Physical Chemistry. Microneedles and their applications. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Soc., 16, 3A, 426-433. PMC Privacy Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Before Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Bohdanecky, Miloslav (1996). Human skins (Full-thickness human skin) were supplied by HansBiomed. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. Microporation applications for enhancing drug delivery. Proc of SPIE. They concluded that a power law behavior seems to be approached only in the small molecular weight region. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. (2003). Careers. PubMedGoogle Scholar. (2008). Springer Nature. Search Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). 2010;4(1):117. On the molecular weight distribution of dextran T-500. Comparison of single-point and dilution procedures. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Biomaterials, 22, 1891-1898. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish The Transdermal diffusion cell system was protected from light. Analytical Chemistry. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. It is well tolerated on parenteral infusion by most people but a very low incidence The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. ; Rani, K.Y. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Biological skin substitutes for wound cover and closure. ; Ghosh, T.K. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden.
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